Diverse effects of overexpression of LEAFY and PTLF, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis.
Identifieur interne : 004814 ( Main/Exploration ); précédent : 004813; suivant : 004815Diverse effects of overexpression of LEAFY and PTLF, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis.
Auteurs : W H Rottmann [États-Unis] ; R. Meilan ; L A Sheppard ; A M Brunner ; J S Skinner ; C. Ma ; S. Cheng ; L. Jouanin ; G. Pilate ; S H StraussSource :
- The Plant journal : for cell and molecular biology [ 0960-7412 ] ; 2000.
Descripteurs français
- KwdFr :
- ADN complémentaire (MeSH), Arbres (génétique), Données de séquences moléculaires (MeSH), Facteurs de transcription (MeSH), Génotype (MeSH), Hybridation in situ (MeSH), Protéines bactériennes (génétique), Protéines d'Arabidopsis (MeSH), Protéines membranaires (génétique), Protéines végétales (génétique), Réaction de polymérisation en chaîne (MeSH), Similitude de séquences d'acides aminés (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- génétique : Arbres, Protéines bactériennes, Protéines membranaires, Protéines végétales.
- ADN complémentaire, Données de séquences moléculaires, Facteurs de transcription, Génotype, Hybridation in situ, Protéines d'Arabidopsis, Réaction de polymérisation en chaîne, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Arabidopsis Proteins (MeSH), Bacterial Proteins (genetics), Base Sequence (MeSH), DNA, Complementary (MeSH), Genotype (MeSH), In Situ Hybridization (MeSH), Membrane Proteins (genetics), Molecular Sequence Data (MeSH), Plant Proteins (genetics), Plants, Genetically Modified (MeSH), Polymerase Chain Reaction (MeSH), Sequence Homology, Amino Acid (MeSH), Transcription Factors (MeSH), Trees (genetics).
- MESH :
- chemical , genetics : Bacterial Proteins, Membrane Proteins, Plant Proteins.
- chemical : Arabidopsis Proteins, DNA, Complementary, Transcription Factors.
- genetics : Trees.
- Amino Acid Sequence, Base Sequence, Genotype, In Situ Hybridization, Molecular Sequence Data, Plants, Genetically Modified, Polymerase Chain Reaction, Sequence Homology, Amino Acid.
Abstract
PTLF, the Populus trichocarpa homolog of LEAFY (LFY) and FLORICAULA, was cloned to assess its function in a dioecious tree species. In situ hybridization studies showed that the gene was expressed most strongly in developing inflorescences. Expression was also seen in leaf primordia and very young leaves, most notably in apical vegetative buds near inflorescences, but also in seedlings. Although ectopic expression of the PTLF cDNA in Arabidopsis accelerated flowering, only one of the many tested transgenic lines of Populus flowered precociously. The majority of trees within a population of 3-year-old transgenic hybrid Populus lines with PTLF constitutively expressed showed few differences when compared to controls. However, phenotypic effects on growth rate and crown development, but not flowering, were seen in some trees with strong PTLF expression and became manifest only as the trees aged. Competence to respond to overexpression of LFY varied widely among Populus genotypes, giving consistent early flowering in only a single male P. tremula x P. tremuloides hybrid and causing gender change in another hybrid genotype. PTLF activity appears to be subject to regulation that does not affect heterologously expressed LFY, and is dependent upon tree maturation. Both genes provide tools for probing the mechanisms of delayed competence to flower in woody plants.
DOI: 10.1046/j.1365-313x.2000.00734.x
PubMed: 10849341
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Pilate</name></author><author><name sortKey="Strauss, S H" sort="Strauss, S H" uniqKey="Strauss S" first="S H" last="Strauss">S H Strauss</name></author></analytic><series><title level="j">The Plant journal : for cell and molecular biology</title><idno type="ISSN">0960-7412</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Arabidopsis Proteins (MeSH)</term><term>Bacterial Proteins (genetics)</term><term>Base Sequence (MeSH)</term><term>DNA, Complementary (MeSH)</term><term>Genotype (MeSH)</term><term>In Situ Hybridization (MeSH)</term><term>Membrane Proteins (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Transcription Factors (MeSH)</term><term>Trees (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (MeSH)</term><term>Arbres (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Facteurs de transcription (MeSH)</term><term>Génotype (MeSH)</term><term>Hybridation in situ (MeSH)</term><term>Protéines bactériennes (génétique)</term><term>Protéines d'Arabidopsis (MeSH)</term><term>Protéines membranaires (génétique)</term><term>Protéines végétales (génétique)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Membrane Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Arabidopsis Proteins</term><term>DNA, Complementary</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arbres</term><term>Protéines bactériennes</term><term>Protéines membranaires</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Genotype</term><term>In Situ Hybridization</term><term>Molecular Sequence Data</term><term>Plants, Genetically Modified</term><term>Polymerase Chain Reaction</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Données de séquences moléculaires</term><term>Facteurs de transcription</term><term>Génotype</term><term>Hybridation in situ</term><term>Protéines d'Arabidopsis</term><term>Réaction de polymérisation en chaîne</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">PTLF, the Populus trichocarpa homolog of LEAFY (LFY) and FLORICAULA, was cloned to assess its function in a dioecious tree species. In situ hybridization studies showed that the gene was expressed most strongly in developing inflorescences. Expression was also seen in leaf primordia and very young leaves, most notably in apical vegetative buds near inflorescences, but also in seedlings. Although ectopic expression of the PTLF cDNA in Arabidopsis accelerated flowering, only one of the many tested transgenic lines of Populus flowered precociously. The majority of trees within a population of 3-year-old transgenic hybrid Populus lines with PTLF constitutively expressed showed few differences when compared to controls. However, phenotypic effects on growth rate and crown development, but not flowering, were seen in some trees with strong PTLF expression and became manifest only as the trees aged. Competence to respond to overexpression of LFY varied widely among Populus genotypes, giving consistent early flowering in only a single male P. tremula x P. tremuloides hybrid and causing gender change in another hybrid genotype. PTLF activity appears to be subject to regulation that does not affect heterologously expressed LFY, and is dependent upon tree maturation. Both genes provide tools for probing the mechanisms of delayed competence to flower in woody plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10849341</PMID><DateCompleted><Year>2000</Year><Month>07</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0960-7412</ISSN><JournalIssue CitedMedium="Print"><Volume>22</Volume><Issue>3</Issue><PubDate><Year>2000</Year><Month>May</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>Diverse effects of overexpression of LEAFY and PTLF, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis.</ArticleTitle><Pagination><MedlinePgn>235-45</MedlinePgn></Pagination><Abstract><AbstractText>PTLF, the Populus trichocarpa homolog of LEAFY (LFY) and FLORICAULA, was cloned to assess its function in a dioecious tree species. In situ hybridization studies showed that the gene was expressed most strongly in developing inflorescences. Expression was also seen in leaf primordia and very young leaves, most notably in apical vegetative buds near inflorescences, but also in seedlings. Although ectopic expression of the PTLF cDNA in Arabidopsis accelerated flowering, only one of the many tested transgenic lines of Populus flowered precociously. The majority of trees within a population of 3-year-old transgenic hybrid Populus lines with PTLF constitutively expressed showed few differences when compared to controls. However, phenotypic effects on growth rate and crown development, but not flowering, were seen in some trees with strong PTLF expression and became manifest only as the trees aged. Competence to respond to overexpression of LFY varied widely among Populus genotypes, giving consistent early flowering in only a single male P. tremula x P. tremuloides hybrid and causing gender change in another hybrid genotype. PTLF activity appears to be subject to regulation that does not affect heterologously expressed LFY, and is dependent upon tree maturation. Both genes provide tools for probing the mechanisms of delayed competence to flower in woody plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rottmann</LastName><ForeName>W H</ForeName><Initials>WH</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Richardson Hall 321, College of Forestry, Oregon State University, Corvallis, OR 97331-5752, USA. de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meilan</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Sheppard</LastName><ForeName>L A</ForeName><Initials>LA</Initials></Author><Author ValidYN="Y"><LastName>Brunner</LastName><ForeName>A M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Skinner</LastName><ForeName>J S</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Jouanin</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Pilate</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>S H</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant J</MedlineTA><NlmUniqueID>9207397</NlmUniqueID><ISSNLinking>0960-7412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis 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PubStatus="pubmed"><Year>2000</Year><Month>6</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>7</Month><Day>15</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>6</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10849341</ArticleId><ArticleId IdType="pii">tpj734</ArticleId><ArticleId IdType="doi">10.1046/j.1365-313x.2000.00734.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><noCountry><name sortKey="Brunner, A M" sort="Brunner, A M" uniqKey="Brunner A" first="A M" last="Brunner">A M Brunner</name><name sortKey="Cheng, S" sort="Cheng, S" uniqKey="Cheng S" first="S" last="Cheng">S. Cheng</name><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L" last="Jouanin">L. Jouanin</name><name sortKey="Ma, C" sort="Ma, C" uniqKey="Ma C" first="C" last="Ma">C. Ma</name><name sortKey="Meilan, R" sort="Meilan, R" uniqKey="Meilan R" first="R" last="Meilan">R. Meilan</name><name sortKey="Pilate, G" sort="Pilate, G" uniqKey="Pilate G" first="G" last="Pilate">G. Pilate</name><name sortKey="Sheppard, L A" sort="Sheppard, L A" uniqKey="Sheppard L" first="L A" last="Sheppard">L A Sheppard</name><name sortKey="Skinner, J S" sort="Skinner, J S" uniqKey="Skinner J" first="J S" last="Skinner">J S Skinner</name><name sortKey="Strauss, S H" sort="Strauss, S H" uniqKey="Strauss S" first="S H" last="Strauss">S H Strauss</name></noCountry><country name="États-Unis"><region name="Oregon"><name sortKey="Rottmann, W H" sort="Rottmann, W H" uniqKey="Rottmann W" first="W H" last="Rottmann">W H Rottmann</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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